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gfp tagged mitf  (Proteintech)


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    Proteintech gfp tagged mitf
    Gfp Tagged Mitf, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+tagged+mitf/MITF+Antibody/pmc07427332__mmc7-319-5-11
    Average 95 stars, based on 47 article reviews
    gfp tagged mitf - by Bioz Stars, 2026-11
    95/100 stars

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    Purification:

    Article Title: Tuning Transcription Factor Availability through Acetylation-Mediated Genomic Redistribution
    Article Snippet: .. GFP- and HIS-tag pull down GFP-tagged MITF were purified using GFP-trap (Chromotek Cat#gtma-100) from transiently transfected Phoenix-ampho with indicated plasmids (total 6 mg DNA in 6 cm dish) using Fugene 6. .. The cells were lysed in 500 mL RIPA supplemented with 5 mM M344 (Stratech Scientific Cat#S2779-SEL) and 4xPIC (Sigma Cat#5892988001) on ice 30 mins before passing through 25 guage Molecular Cell 79, 472–487.e1–e10, August 6, 2020 e6 ll OPEN ACCESS Article needle for mechanical disruption till no visible clump is seen follow by pre-clearing at 14,000 x g centrifugation for 10 mins.

    Article Title: Tuning Transcription Factor Availability through Acetylation-Mediated Genomic Redistribution
    Article Snippet: .. GFP-tagged MITF were purified using GFP-trap (Chromotek Cat#gtma-100) from transiently transfected Phoenix-ampho with indicated plasmids (total 6 μg DNA in 6 cm dish) using Fugene 6. .. The cells were lysed in 500 μL RIPA supplemented with 5 μM M344 (Stratech Scientific Cat#S2779-SEL) and 4xPIC (Sigma Cat#5892988001) on ice 30 mins before passing through 25 guage needle for mechanical disruption till no visible clump is seen follow by pre-clearing at 14,000 x g centrifugation for 10 mins.

    Transfection:

    Article Title: Tuning Transcription Factor Availability through Acetylation-Mediated Genomic Redistribution
    Article Snippet: .. GFP- and HIS-tag pull down GFP-tagged MITF were purified using GFP-trap (Chromotek Cat#gtma-100) from transiently transfected Phoenix-ampho with indicated plasmids (total 6 mg DNA in 6 cm dish) using Fugene 6. .. The cells were lysed in 500 mL RIPA supplemented with 5 mM M344 (Stratech Scientific Cat#S2779-SEL) and 4xPIC (Sigma Cat#5892988001) on ice 30 mins before passing through 25 guage Molecular Cell 79, 472–487.e1–e10, August 6, 2020 e6 ll OPEN ACCESS Article needle for mechanical disruption till no visible clump is seen follow by pre-clearing at 14,000 x g centrifugation for 10 mins.

    Article Title: Tuning Transcription Factor Availability through Acetylation-Mediated Genomic Redistribution
    Article Snippet: .. GFP-tagged MITF were purified using GFP-trap (Chromotek Cat#gtma-100) from transiently transfected Phoenix-ampho with indicated plasmids (total 6 μg DNA in 6 cm dish) using Fugene 6. .. The cells were lysed in 500 μL RIPA supplemented with 5 μM M344 (Stratech Scientific Cat#S2779-SEL) and 4xPIC (Sigma Cat#5892988001) on ice 30 mins before passing through 25 guage needle for mechanical disruption till no visible clump is seen follow by pre-clearing at 14,000 x g centrifugation for 10 mins.



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